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Aim: The study aimed to evaluate the protective effect of Citrus aurantiifolia against cadmium chloride toxicity induced in the liver and kidney of Albino Wistar rats histologically.
Methods: Twenty five adult female Albino rats weighing 150±11 to 200±50 g were used for the study. The rats were purchased from the Department of Pharmacology, College of Health Science, University Port Harcourt, Rivers State of Nigeria. They were randomly assigned into five groups (A B C D& E) with each group having five rats. Group A (Control) received only food and water for six weeks (6 weeks), Group B received food, cadmium water and treatment with lime juice for six weeks (6 weeks), Group C received food, water and lime juice for three weeks. After three weeks, they stopped lime and water and were induced with cadmium chloride for 3 weeks, Group D received food, cadmium chloride for 3 weeks and after 3 week they were given normal water and treated with lime for another 3 weeks, Group E received food and cadmium water for six weeks. At the end of the treatment, the liver and the kidney of each sacrificed rat were processed for paraffin sectioning and stained with Harris hematoxylin and eosin.
Results: Photomicrographs of Groups B and C which were induced with Cadmium Chloride and treated with citrus shows area of central necrosis and central vein congested with red blood cells and also the presence of inflammatory cells which are features of liver injury can be seen.
Conclusion: There was no significant protective effect of Citrus aurantiifolia against cadmium-induced liver injury in Albino Wistar rats. Also, there was no significant effect of cadmium-induced toxicity on the kidney of the Albino Wistar rat.
Aneta Salińska, Tadeusz Włostowski, Ewa Oleńska. Differential susceptibility to cadmium-induced liver and kidney injury in wild and laboratory-bred bank voles Myodes glareolus. US National Library of Medicine National Institutes of Health. Archives of Environmental Contamination and Toxicology. 2013;65(2):324–331.
Kuester RK, Waalkes MP, Goering PL, Fisher BL, McCuskey RS, Sipes IG. Differential hepatotoxicity induced by cadmium in Fischer 344 and Sprague-Dawley rats. An Official Journal of the Society of Toxicology and Science. 2002; 65:151–159.
[PubMed] [Cross Ref]
Salińska A, Włostowski T, Oleńska E. Differential susceptibility to cadmium-induced liver and kidney injury in wild and laboratory-bred bank voles Myodes glareolus. Archives of Environmental Contamination and Toxicology. 2013;65(2): 324-331.
Liu J, Kershaw WC, Liu Y, Klaassen CD. Cadmium-induced hepatic endothelial cell injury in inbred strains of mice. Archives of Environmental Contamination and Toxicology. 1992;75:51–62.
Włostowski T, Krasowska A, Łaszkiewicz-Tiszczenko B. Dietary cadmium induces histopathological changes despite a sufficient metallothionein level in the liver and kidneys of the bank vole (Clethrionomys glareolus). Archives of Environmental Contamination and Toxicology. 2001;126:21–28.
Sabolić I, Breljak D, Škarica M, Herak-Kramberger CM. Role of metallothionein in cadmium traffic and toxicity in kidneys and other mammalian organs. Biometals: An International Journal on the role of Metal Ions in Biology, Biochemistry and Medicine. 2010;23:897–926.
Shrestha RL, Dhakal DD, Gautum DM, Paudyal KP, Shrestha S. Variation of physiochemical components of acid lime (Citrus aurantiifolia swingle) fruits at different sides of the tree in Nepal. American Journal of Plant Science. 2012; 3:1688-1692.
Karoui IJ, Marzouk M. Characterization of bioactive compounds in tunisian bitter orange (Citrus aurantium L.) peel and juice and determination of their antioxidant activities. Journal of Bio Medical Research International. 2013;10: 115-345
Patil J, Chidambara R, Murthy KN, Jayaprakasha GK, Chetti MB, Patil BS. Bioactive compounds from Mexican lime (Citrus aurantiifolia) juice induce apoptosis in human pancreatic cells. Journal of Agriculture and Food Chemistry. 2009; 57(22):10933-42.
Gurer H, Ozgunes H, Neal R, Spitz DR. Ercal N. Antioxidant effects of N-acetylcysteine and succimer in red blood cells from lead-exposed rats. Archives of Environmental Contamination and Toxicology. 1998;128:181-189.
Obianime AW, Ahiwe NJ, Aprioku JS. Effects of vitamins C and E pretreatments on cadmium-induced serum levels of some biochemical and hormonal parameters in the female guinea pig. African Journal of Biotechnology. 2010;9: 6582-6587.
Sajitha GR, Jose R, Andrews A, Ajantha KG, Augustine P, Augusti KT. Garlic oil and vitamin E prevent the adverse effects of lead acetate and ethanol separately as well as in combination in the drinking water of rats. Indian Journal of Clinical. Biochemistry. 2010;25:280-288.
Ayinde OC, Ogunnowo S, Ogedegbe RA. Influence of vitamin C and vitamin E on testicular zinc content and testicular toxicity in lead exposed Albino rats. BMC Pharmacology and Toxicology. 2012;13.
National Research Council. Guide for the Care and Use of Laboratory Animals. 8th ed. Washington: The National Academies Press; 2011.
Weerachet J, Nattapong S, Sakornpob N. The comparison between the quality of lime juice produced by different preservation techniques. Procedia Social and Behavioral Science. 2013;91:691-696.
Amal I El-Refaiy, Fawzy I. Histopathology and cytotoxicity as biomarkers in treated rats with cadmiumand some therapeutic agents.Saudi Journal Bio Sci. 2013;20(3): 265-280.
Jarup LA, Rogenfelt CG, Elinder, Biological half-time of cadmium in the blood of workers after cessation of exposure. Scandinavian Journal of Work, Environment and Health. 1983;9(4):327–331. View at Google Scholar.