Antimalarial Activity of Ethanol Extract of Mucuna pruriens Leaves on Nk65 Chloroquine Sensitive Strain of Plasmodium berghei

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O. E. Ezim
O. V. Alagbe
F. M. Idih


Mucuna pruriens leaves are used in some part of Nigeria for the treatment of malaria and anemia. With an estimated 3.3 billion people in 97 countries and territories at risk of being infected with malaria according to the WHO, researching into new chemotherapeutic agent against this disease is indeed necessary. This study was designed to evaluate the antimalarial effect of ethanol extract of Mucuna pruriens leaves on NK65 chloroquine sensitive strain of plasmodium berghei in mice. The bioactive compounds in the extract were identified using GC-MS. The experimental animals were divided into 6 groups: negative control, normal control, groups treated with chloroquine (10 mg/kg), Artemeter/Lumefantrine-ACT (20 mg/120 mg/kg), 500 mg/kg of M. pruriens, 1000 mg/kg of M. pruriens and 2000 mg/kg of M. pruriens respectively. Parasite inoculation was done by intraperitoneal injection of 0.2ml of the inoculum (1×107 infected erythrocytes). The GCMS result revealed the extract contains n-hexadeconoic acid, a compound known to possess antimalarial properties. The study revealed that the administrations M. pruriens leaves extracts at suitable doses reduced the parasite load and were able to maintain the PCV at a normal range with a stabilising effect on body weight.

Antimalarial, parasitemia, Mucuna pruriens, bioactive compounds, alternative medicine

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How to Cite
Ezim, O. E., Alagbe, O. V., & Idih, F. M. (2021). Antimalarial Activity of Ethanol Extract of Mucuna pruriens Leaves on Nk65 Chloroquine Sensitive Strain of Plasmodium berghei. Journal of Complementary and Alternative Medical Research, 13(4), 1-7.
Original Research Article


Farnswort NR, Akerele O, Bingel AS, Soejarto DD, Guo Z. Bulletin WHO. 1985; 63:965-972.

Akindele AJ, Busayo FI. Effects of the hydroethanolic extract of Mucuna pruriens (L) DC (Fabaceae) on haematological profile in normal and haloperidol treated rats. Nig Q J Hosp Med. 2011; 21(2):93-8.

Mikhail ON, Taoheed AA, Musbau AA. Phytochemical analysis and antimalarial activity of aqueous extract of Lecaniodiscus cupanioides root. Journal of Tropical Medical. 2013;605393.

David LH. Methods of hematology. Sweet Haven Publishing Service; 2014.

Monica C. Hematological test: Blood films. District Laboratory Practice in Tropical Countries. 2005;8(7).

Menendez C, Fleming AF, Alonso PL. Malaria-related anaemia. Parasitology today. 2000;16(11):469-476.

Basir R, Fazalul R, Hasballah K, Chong WC, Talib H, Yam FM, Jabbarzare M, Tie TH, Othman F, Moklas MAM, Abdullah WO, Ahmad Z. Plasmodium berghei ANKA infection in ICR mice as a model of cerebral malaria. Iranian Journal of Parasitology. 2012;7(4):62-74.

Odeghe OB, Uwakwe AA, Monago CC. Antiplasmodial activity of methanolic stem bark extract of Anthocleista grandiflora in mice. International Journal of Applied Science and Technology. 2012;2(4):142-148.

Joseph AB, Oyindamola OA, Olugbenga A, Christian TH, Akintude S, Grace OG. Interaction between rifampicin, amidiaquinine and atemether in mice infected with chloroquine resistant Plasmodium berghei. Malaria Journal. 2014;13:299.