Journal of Complementary and Alternative Medical Research 2019-11-14T07:34:51+00:00 Journal of Complementary and Alternative Medical Research Open Journal Systems <p style="text-align: justify;"><strong>Journal of Complementary and Alternative Medical Research (ISSN: 2456-6276)</strong> aims to publish high quality papers (<a href="/index.php/JOCAMR/general-guideline-for-authors">Click here for Types of paper</a>) in the areas of Complementary, Alternative and Integrative medical research. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.</p> Hydroethanolic Extract of Buchholzia coriacea Seeds Alleviates LPS Induced Liver Injury in Rat via Antioxidant and Anti-inflammatory Actions 2019-11-14T07:34:51+00:00 Ayokanmi Ore Regina Ngozi Ugbaja Abideen Idowu Adeogun Oluseyi Adeboye Akinloye <p><strong>Aim:</strong> This study evaluates the hepatoprotective effects of hydroethanolic extract of (defatted) <em>Buchholzia coriacea</em> seed (HEBCS) against lipopolysaccharide (LPS) induced inflammatory liver injury in the rat.</p> <p><strong>Method:</strong> Thirty male albino rats (120–130 g) were assigned into five groups (n=6/ group). Group I (Control) and II (LPS) received distilled water orally (p.o.) for seven days while animals in groups III, IV and V were administered HEBCS at 125, 250 and 500 mg/kg body weight (b.w.) p.o. daily for 7 days. LPS was injected on the 7th day at a single dose of 4 mg/kg intraperitoneally (i.p.) to the rats in group II, and groups III, IV and V. Six hours after LPS injection, blood was collected to prepare serum, and liver was removed for preparation of homogenate, histopathology and immunofluorescence staining. Biomarkers of liver function (ALT, AST and ALP) and oxidative stress (NO, SOD, CAT and MDA) were assessed. Pro-inflammatory cytokine (TNF-α) and inducible protein (C-reactive protein) were also assessed.</p> <p><strong>Results:</strong> Results showed significant amelioration of liver function markers and levels of pro-inflammatory proteins by HEBCS. Histopathological studies showed a reduction in inflammatory cells and improvement in liver structure in animals treated with HEBCS.</p> <p><strong>Conclusion:</strong> Data from the current study show that HEBCS possesses hepatoprotective effects against LPS induced inflammatory liver injury.</p> 2019-10-14T00:00:00+00:00 ##submission.copyrightStatement## The Significance of Traditional Medicinal Plants Used for Treating Non-communicable Disease by Kani Tribes in Papanasam, Tirunelveli District 2019-11-14T07:34:51+00:00 R. Sathiya A. Manoharan A. Rajarajeshwari <p>The significance of medicinal plants used by the ethnic group of people (<em>Kani/Kanikaran</em>) of Karaiyar, Papanasam through an ethnobotanical survey for treating Non-communicable diseases (NCD) is documented. Data were collected through a questionnaire survey. Sample size in the survey covers 30 tribal people (men-13, women-17) to analyse various factors and their relationship to the diseases. &nbsp;The survey has included the age range, diseases frequency, gender stats, occupation, BMI, habits and the medicinal practices used. It is estimated that 67% of people still use traditional means for the treatment of diseases. Major medicinal plants used are indigenous to their geographical area which revealed high esteem of ethnobotanical significance and proven to be an effective and sustainable means of treating Non-communicable diseases.</p> 2019-10-18T00:00:00+00:00 ##submission.copyrightStatement## Therapeutic Effects of the Anti-diabetic Polyherbal Drug Diawell in Combination with Metformin on Liver and Lipid Parameters in Type 2 Diabetic Rats 2019-11-14T07:34:50+00:00 O. N. Briggs E. O. Nwachuku H. Brown K. N. Elechi-Amadi <p>Type 2 diabetes is one of the most important diseases worldwide. It affects several organ systems including the liver and lipid metabolism. Many herbal formulations have shown anti-diabetic potential, however, their safety and efficacy remain a debate in the medical community.</p> <p><strong>Aim:</strong> This study evaluates the therapeutic effects of the anti-diabetic polyherbal drug diawell in combination with metformin on liver enzyme and lipid profile in type 2 diabetic rats.</p> <p><strong>Methodology:</strong> A total of 35 male Wistar albino rats weighing between 120-220 g were used for this study. The rats were placed on high fat diet, and diabetes was induced by a single intraperitoneal injection of freshly prepared streptozotocin (STZ) (45 mg/kg body wt). Fasting plasma glucose (FPG) was determined using the glucose oxidase method. Total Cholesterol (TC), Triglyceride (TG) and High density lipoprotein cholesterol (HDL-C) were determined using enzymatic methods. Low density lipoprotein cholesterol (LDL-C) was calculated using the Friedewald equation. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined using Reitman-Frankel method, while alkaline phosphatase (ALP) was determined using the colorimetric phenolphthalein method. Liver sections were stained using haematoxylin and eosin (H&amp;E) staining technique, and phytochemical analysis was also done on the herbal tablet.</p> <p><strong>Results: </strong>The results show no significant differences in mean TC levels in all groups. TG level was significantly higher in the diabetic control when compared to the negative control. There were no significant differences in TG levels in the metformin group, and diawell group when compared to the diabetic control. TG levels in the combination group (metformin + diawell) was significantly lower versus the diabetic control, and showed no significant difference compared to the negative control. HDL-C was significantly higher in the negative control when compared to the diabetic control and the treatment groups. There were no significant differences in HDL-C levels in all the treatment groups, when compared to the diabetic control. LDL-C levels were significantly lower in the negative control compared to the diabetic control and treatment groups. There were no significant differences in LDL-C levels in all the treatment groups, when compared to the diabetic control. The diabetic control had significantly higher ALT, AST and ALP levels compared to the negative control and treatment groups. All the treatment groups showed no significant differences in ALT and AST levels compared to the negative control. Liver sections of the negative control showed normal histoarchitecture. The diabetic control showed inflammation and fatty deposition. The treatment groups showed a nearly normal histoarchitecture, with fatty deposits.</p> <p><strong>Conclusion:</strong> High fat diet in combination with 45 mg/kg of STZ produced significant diabetes in the Wistar rats with dyslipidaemia and elevated liver enzyme levels. Metformin and the polyherbal tablet diawell had no impact on the lipid levels as it did not correct the dyslipidaema, however, the treatments showed hepatoprotective potentials and restored liver enzyme levels to normal. Lipid lowering drugs should be included in the management of type 2 diabetes, and there should be proper evaluation of anti-diabetic herbal products.</p> 2019-10-28T00:00:00+00:00 ##submission.copyrightStatement##