Hydroethanolic Extract of Buchholzia coriacea Seeds Alleviates LPS Induced Liver Injury in Rat via Antioxidant and Anti-inflammatory Actions
Ayokanmi Ore *
Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta, Nigeria and Biochemistry Division, Department of Chemical Sciences, Faculty of Natural Sciences, Ajayi Crowther University, Oyo, Nigeria.
Regina Ngozi Ugbaja
Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta, Nigeria.
Abideen Idowu Adeogun
Department of Chemistry, College of Physical Sciences, Federal University of Agriculture, Abeokuta, Nigeria.
Oluseyi Adeboye Akinloye
Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Aim: This study evaluates the hepatoprotective effects of hydroethanolic extract of (defatted) Buchholzia coriacea seed (HEBCS) against lipopolysaccharide (LPS) induced inflammatory liver injury in the rat.
Method: Thirty male albino rats (120–130 g) were assigned into five groups (n=6/ group). Group I (Control) and II (LPS) received distilled water orally (p.o.) for seven days while animals in groups III, IV and V were administered HEBCS at 125, 250 and 500 mg/kg body weight (b.w.) p.o. daily for 7 days. LPS was injected on the 7th day at a single dose of 4 mg/kg intraperitoneally (i.p.) to the rats in group II, and groups III, IV and V. Six hours after LPS injection, blood was collected to prepare serum, and liver was removed for preparation of homogenate, histopathology and immunofluorescence staining. Biomarkers of liver function (ALT, AST and ALP) and oxidative stress (NO, SOD, CAT and MDA) were assessed. Pro-inflammatory cytokine (TNF-α) and inducible protein (C-reactive protein) were also assessed.
Results: Results showed significant amelioration of liver function markers and levels of pro-inflammatory proteins by HEBCS. Histopathological studies showed a reduction in inflammatory cells and improvement in liver structure in animals treated with HEBCS.
Conclusion: Data from the current study show that HEBCS possesses hepatoprotective effects against LPS induced inflammatory liver injury.
Keywords: Lipopolysaccharide, Buchholzia coriacea, liver, antioxidants, inflammation, immunofluorescence, rat.