Hepatoprotective Potential of Hura crepitans L.: A Review of Ethnomedical, Phytochemical and Pharmacological Studies

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Oluwole S. Owojuyigbe
Caleb K. Firempong
Christopher Larbie
Gustav Komlaga
Benjamin O. Emikpe


Herbal medicines are the main source of treatment of diseases in non-urban centres of the developing world. Secondary metabolites obtained from herbal sources contain bioactive phytochemicals, many of which have been the origin for the development of novel pharmaceutical drugs. Hura crepitans L. (Euphorbiaceae) or sandbox tree has been beneficial in many ethnomedicinal applications as a purgative, emetic, hepatoprotective, anti-inflammatory, antimicrobial and the treatment of leprosy. Toxicological, phytochemical and bactericidal studies have also been documented. This paper reviews the potential of the Hura crepitans plant in protecting the liver against drug-induced toxicity. The paper dwells extensively on the ethnomedical, phytochemical and pharmacological properties of the plant. In achieving the above, intensive analyses of books and published peer-reviewed journal articles were carried out using credible scientific databases. Four main phytochemicals were revealed to be contained in Hura crepitans stem-bark. Their protective effects were enunciated using animal models. However much more biochemical studies need to be done to establish the hepatoprotective potentials of the various parts and various phytochemicals of Hura crepitans with the need for more preclinical and clinical studies. We, therefore, present in this paper efforts to elucidate and bring to the fore the therapeutic potentials of Hura crepitans plant.

Hura crepitans L., toxicological assessment, phytochemical properties, hepato-protection

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How to Cite
Owojuyigbe, O. S., Firempong, C. K., Larbie, C., Komlaga, G., & Emikpe, B. O. (2020). Hepatoprotective Potential of Hura crepitans L.: A Review of Ethnomedical, Phytochemical and Pharmacological Studies. Journal of Complementary and Alternative Medical Research, 9(2), 1-10. https://doi.org/10.9734/jocamr/2020/v9i230136
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Madrigal-Santillán E, Madrigal-Bujaidar E, Álvarez-González I, Sumaya-Martínez MT, Gutiérrez-Salinas J, Bautista M, Morales-González A, González-Rubio M, Aguilar-Faisal JL, Morales-González JA. Natural products with hepatoprotective effects. World J. Gastroenterol. 2014;20(40): 14787–14804.

Marcellin P, Kutala BK. Liver diseases: A major, neglected global public health problem requiring urgent actions and large-scale screening. Liver Int. 2018;38:2–6.

Floreani A, De Martin S, Secchi MF, Cazzagon N. Extrahepatic autoimmunity in autoimmune liver disease. Eur. J. Intern. Med. 2019;59:1–7.

Baecker A, Liu X, La Vecchia C, Zhang ZF. Worldwide incidence of hepatocellular carcinoma cases attributable to major risk factors. Eur. J. Cancer Prev. 2018;27(3): 205-212.

Younossi ZM, Stepanova M, Afendy M, Fang Y, Younossi Y, Mir H, Srishord M. Changes in the prevalence of the most common causes of chronic liver diseases in the United States from 1988 to 2008. Clin. Gastroenterol. H. 2011;9(6):524-530. e521.

Teschke R. Alcoholic liver disease: Alcohol metabolism, cascade of molecular mechanisms, cellular targets and clinical aspects. Biomedicines. 2018;6:106.

Bataller R, Arteel GE, Moreno C, Shah V. Alcohol-related liver disease: Time for action. J. Hepatol. 2019;70(2):221-222.

Omabe M, Omabe K, Igwe D, John O, Uchenna S, Elom S. Xenobiotics-induced liver damage is biochemically abrogated by treatment with lipophilic extracts of Moringa oleifera In vivo. Health. 2018;10: 313-325.

Latha TB, Srikanth A, Eswar KK, Mastan K, Srinivasa RY, Bhavani B. Comparative hepatoprotective efficacy of kumaryasava and livfit against carbon tetrachloride induced hepatic damage in rats. Pharmacologyonline. 2009;1:1127-1134.

Asrani SK, Devarbhavi H, Eaton J, Kamath PS. Burden of liver diseases in the world. J. Hepatol. 2019;70(1):151-171.

Spearman CW, Sonderup MW. Health disparities in liver disease in sub- Saharan Africa. Liver Int. 2015;35(9):2063-71.

Levy SB. The challenge of antibiotic resistance. Sci Am. 1998;278:32-39.

Smolinski MS, Hamburg MA, Lederberg J. Microbial threats to health: Emergence, detection and response. Washington, DC: Institute of Medicine, National Academies Press. 2003;203-210.

Orhan DD, Orhan N, Ergun E, Ergun F. Hepatoprotective effect of Vitis vinifera L. leaves on carbon tetrachloride-induced acute liver damage in rats. J. Ethnopharmacol. 2007;112(1):145-151.

Rai, M. Herbal medicines in India: Retrospect and prospect. Fitoterapia. 1994;65:483-491.

Schuppan D, Jia JD, Brinkhaus B, Hahn EG. Herbal products for liver diseases: A therapeutic challenge for the new millennium. Hepatology. 1999;30(4):1099-1104.

Subramoniam A, Pushpangadan P. Development of phytomedicines for liver disease. Indian J. Pharmacol. 1999;31(3): 166.

Akindele, AJ, Ezenwanebe, KO, Anunobi, CC, Adeyemi, OO. Hepatoprotective and in vivo antioxidant effects of Byrsocarpus coccineus Schum. and Thonn. (Connaraceae). J. Ethnopharmacol. 2010; 129(1):46-52.

Amat N, Upur H, Blažeković, B. In vivo hepatoprotective activity of the aqueous extract of Artemisia absinthium L. against chemically and immunologically induced liver injuries in mice. J. Ethnopharmacol. 2010;131(2):478-484.

Bishayee A, Sarkar A, Chatterjee M. Hepatoprotective activity of carrot (Daucus carota L.) against carbon tetrachloride intoxication in mouse liver. J. Ethnopharmacol. 1995;47(2):69-74.

Suja S, Latha P, Pushpangadan P, Rajasekharan S. Evaluation of hepato-protective effects of Helminthostachys zeylanica (L.) Hook against carbon tetrachloride-induced liver damage in Wistar rats. J. Ethnopharmacol. 2004; 92(1):61-66.

Opoku A, Ndlovu I, Terblanche S, Hutchings A. In vivo hepatoprotective effects of Rhoicissus tridentata subsp. cuneifolia, a traditional Zulu medicinal plant, against CCl4-induced acute liver injury in rats. S. Afr. J. Bot. 2007;73(3): 372-377.

Dahiru D, William E, Nadro M. Protective effect of Ziziphus mauritiana leaf extract on carbon tetrachloride-induced liver injury. Afr. J. Biotechnol. 2005;4(10):1177-1179.

Arthur FKN, Woode E, Terlabi EO, Larbie C. Evaluation of hepatoprotective effect of aqueous extract of Annona muricata (Linn.) leaf against carbon tetrachloride and acetaminophen induced liver damage. J. Nat. Pharm. 2012;3(1):25–30.

Larbie C, Torkornoo D, Nyanor E, Asibey O. Evaluation of the hepatoprotective potential of hydroethanolic extract of Ficus pumila L. on CCl4 induced liver damage in rats. Global J. Res. Med. Plants and Indigen. Med. 2016;5(7):217-225.

Sarfo-Antwi F, Larbie C, Duduyemi B. Extracts of Ageratum conyzoides L. protects against carbon tetrachloride – Induced toxicity in rats through inhibiting oxidative stress. J. Advances Med. Pharma. Sci. 2018;19(2):1-14.
DO1: 0.9734/JAMPS/2018/ 45189

Adewusi E, Afolayan A. A review of natural products with hepatoprotective activity. J. Med. Plants Res. 2010;4(13):1318-1334.

Singh A, Handa S. Hepatoprotective activity of Apium graveolens and Hygrophila auriculata against paracetamol and thioacetamide intoxication in rats. J. Ethnopharmacol. 1995;49(3):119-126.

Jafri M, Subhani MJ, Javed K, Singh S. Hepatoprotective activity of leaves of Cassia occidentalis against paracetamol and ethyl alcohol intoxication in rats. J. Ethnopharmacol. 1999;66(3):355-361.

Olaleye M, Adegboye O, Akindahunsi A. Alchornea cordifolia extract protects wistar albino rats against acetaminophen-induced liver damage. Afr. J. Biotechnol. 2006; 5(24).

Post-White J, Ladas EJ, Kelly KM. Advances in the use of milk thistle (Silybum marianum). Integr. Cancer Ther. 2007;6(2):104-109.

Boigk G, Stroedter L, Herbst H, Waldschmidt J, Riecken EO, Schuppan D. Silymarin retards collagen accumulation in early and advanced biliary fibrosis secondary to complete bile duct obliteration in rats. Hepatol. 1997;26(3): 643-649.

Zhao J, Lahiri-Chatterjee M, Sharma Y, Agarwal R. Inhibitory effect of a flavonoid antioxidant silymarin on benzoyl peroxide-induced tumor promotion, oxidative stress and inflammatory responses in Sencar mouse skin. Carcinogenesis 2000;21(4): 811-816.

Crocenzi FA, Roma MG. Silymarin as a new hepatoprotective agent in experimental cholestasis: New possibilities for an ancient medication. Curr. Med. Chem. 2006;13(9):1055-1074.

Halim AB, El-Ahmady O, Abdel-Galil F, Darwish A, Hassab-Allah S. Hafez Y. Biochemical effect of antioxidants on lipids and liver function in experimentally-induced liver damage. Ann. Clin. Biochem. 1997;34(6):656-663.

Valenzuela A, Guerra R. Protective effect of the flavonoid silybin dihemisuccinate on the toxicity of phenylhydrazine on rat liver. FEBS Lett. 1985;181(2):291-294.

Oloyede GK, Adaramoye OA, Olatinwo MB. Chemical constituents of sandbox tree (Hura crepitans Linn.) and anti hepatoxic activity of leaves and stem bark extracts; 2016.
DOI: 10.7727/wimj.2015.247

Burkill HM. The useful plants of west tropical Africa, Vols. 1-3, Royal Botanic Gardens, Kew; 1995.

Oderinde RA, Ajayi I, Adewuyi A. Preliminary toxicological evaluation and effect of the seed oil of Hura crepitans and Blighia unijugata Bark on the lipid profile of Rat. EJEAFChe 2009;8(3):209-217.

Jaffé WG. Hurain. A new plant protease from Hura crepitans. J. Biol. Chem. 1943; 149:1-7.

Kawazu K. Active constituents of piscicidal plants. J. Syn. Org. Chem. Jpn. 1972; 30(7):615-628.

Jernigan KA. Barking up the same tree: A comparison of ethnomedicine and canine ethnoveterinary medicine among the Aguaruna. J. Ethnobiol. Ethnomed. 2009; 5(1):1.

Oloyede GK, Olatinwo MB. Phytochemical investigation, toxicity and antimicrobial screening of essential oil and extracts from leaves and stem bark of Hura crepitans (Euphorbiaceae). Academia Arena. 2014; 6(5):7-15.

Oloyede GK, Olatinwo M. In vitro antioxidant activity of extracts from the leaves of Hura crepitans (Euphorbiaceae)-a comparison of two assay methods. Cell Membranes and Free Radical Research. 2011;3(1):133-138.

Uchiyama C, Ishida K, Tsutsui T, Naito A, Kurita K, Hanihara H, Serizawa T, Fujiwara M, Ohdera M. Effects of Hura crepitans and its active ingredient, daphne factor F3, on dihydrotestosterone-induced neurotrophin-4 activation and hair retardation. Biol. Pharm. Bull. 2012;35(1): 42-47.

Dagang W, Sorg B, Adolf W, Seip EH, Hecker E. Oligo- and macrocyclic diterpenes in thymelaeaceae and euphorbiaceae occurring and utilized in Yunnan (southwest China). Phytother. Res. 1991;5:163-16.

Falasca A, Franceschi A, Rossi C, Stirpe, F. Mitogenic and haemagglutinating properties of a lectin purified from Hura crepitans seeds. Biochimica et Biophysica Acta (BBA)-General Subjects. 1980; 632(1):95-105.

Mattson MP, Cheng A. Neurohormetic phytochemicals: Low-dose toxins that induce adaptive neuronal stress responses. Trends Neurosci. 2006;29(11):632-639.

Uddin SN, Akond M, Mubassara S, Yesmin MN. Antioxidant and antibacterial activities of Trema cannabina. Middle-East J. Sci. Res. 2008;3(2):105-108.

Jayasri M, Mathew L, Radha A. A report on the antioxidant activity of leaves and rhizomes of Costus pictus D. Don. International Journal of Integrative Biology. 2009;5(1):20-26.

Rice-Evans C, Miller N, Paganga G. Antioxidant properties of phenolic compounds. Trends Plant Sci. 1997;2(4): 152-159.

Avoseh NO, Ogunbajo LO, Ogunwande IA, Ogundajo LA, Lawal AO. Anti-inflammatory Activity of Hexane and Ethyl Acetate Extracts of Hura crepitans L. EJMP. 2018; 24(1):1-6. DOI.org/10.9734/EJMP/2018/41439

Fowomola MA, Akindahunsi AA. Nutritional quality of sandbox tree (Hura crepitans Linn.). J. Med. Food. 2007;10(1):159-164.


Adedire MA, Ajayi, OA. Potential of sandbox Hura crepitans seed oil for protection of cowpea seeds from Bruchidae infestation. J. Plant Dis. Protect. 2003;110(6):1-9.

Olatidoye OP, Adeleke AE, Adegbite SA, Sobowale SS. Chemical composition and nutritional evaluation of sandbox (Hura crepitans) seed flour for domestic consumption and industrial utilization in Nigeria. J. Med. Appl. Biosci. 2010;5(2): 125–129.

Abdulkadir MN, Amoo IA, Adesina AO. Chemical composition of Hura crepitans seeds and antimicrobial activities of its oil. Int. J. Sci. Res. 2014;2(3):440-443.

Igwenyi IO, Agu EA, Awoke JN, Edwin N, Famurewa AC, Obasi, NA, Obasi, DC. Antidiabetic and hepatoprotective effect of Hura crepitans seed extract in alloxan-induced diabetic albino rats. Int. J. Biol. Pharm. Allied Sci. 2017;6(9):1771-1780.