Evaluation of Hydro-alcoholic Extract of Clerodendrum myricoides (Hochst. Vatke) Leaves and Its Solvent Fractionsin Pentylenetetrazole-Induced Convulsion in Mice

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Teketel Eristu Kediso
Tesfaye Tolessa
Fikirte Getachew
Eyasu Makonnen
Daniel Seifu

Abstract

Introduction: Epilepsy is a chronic neurological disorder that affects people of all ages. Herbal medicines are widely used across the globe due to their wide applicability and therapeutic efficacy. The low side effects of traditional herbal medicines have encouraged many types of research into antiepileptic activity. Clerodendrum myricoides is a plant whose leaves extract is traditionally used as an anticonvulsant in Ethiopia. 

Objective: The point of this investigation was to assess the anticonvulsant effect of the hydro-alcoholic extract and solvent fractions of C. myricoides leaves against pentylenetetrazole-induced seizures in mice.  

Methods: Comparison of mean latency to onset of convulsion, mean duration of convulsions, and the proportion of percentage protection against seizure of the plant extract was tested against PTZ-induced seizures. Three different doses were used by giving them orally 30 minutes before subcutaneous pentylenetetrazole (80 mg/kg) administration with the positive (diazepam 2 mg/kg) and negative (physiological saline 10 mg/kg) control groups. Data were presented as the mean ± standard error of the mean and analyzed using a one-way analysis of variance (ANOVA) and followed by post-hoc Tukey’s multiple comparisons test. Fisher’s exact test was used for the percentage protection. P < 0.05 was considered statistically significant.

Results: The crude extract of C. myricoides, with the doses of 300, 600, 1,200 mg/kgshowed a significant delay in mean latency to onset of seizures [299.33±30.129 sec (p< 0.05); 387.167± 27.6 sec (p<0.01); 417.833±31.9 sec (p<0.001); respectively)]; decrease in the duration of convulsion [27.333±1.585 sec (p<0.05); 16.833±1.537 sec (p<0.01); 10.50±0.671 sec (p<0.001) respectively]; and a proportion of percentage protection of mice against seizure [16.33% (1/6) (p< 0.05); 33.33% (2/6) (p<0.01); 50% (3/6) (p<0.001) respectively] in a dose-dependent manner compared to the control group [92.833±13.006 sec; 34.167±3.683 sec, 0%  respectively]. C. myricoides anticonvulsant activity was less than that of diazepam [1001.16± 68.430 sec, 4.500±0.619, 83.33 sec, 83.33% respectively for the doses]. Its solvent fractions, however, didn’tshow a significant anticonvulsant effect.

Conclusion: The hydroalcoholic leaves crude extract of C. myricoides has anticonvulsant activity but its solvent fractions do not have comparable significant effects.   

Keywords:
Anticonvulsant effects, hydro-alcoholic extracts, solvent fractions, Clerodendrum myricoides, pentylenetetrazole, mice

Article Details

How to Cite
Kediso, T. E., Tolessa, T., Getachew, F., Makonnen, E., & Seifu, D. (2020). Evaluation of Hydro-alcoholic Extract of Clerodendrum myricoides (Hochst. Vatke) Leaves and Its Solvent Fractionsin Pentylenetetrazole-Induced Convulsion in Mice. Journal of Complementary and Alternative Medical Research, 10(3), 1-10. https://doi.org/10.9734/jocamr/2020/v10i330163
Section
Original Research Article

References

James OM. Drugs effective in the therapy of epilepsy. The pharmacological basis of therapeutics. 2001;10:521-47.

Wannang NN, Anuka JA, Kwanashie HO, Gyang S, Auta A. Anti-seizure activity of the aqueous leaf extract of Solanum nigrum linn (solanaceae) in experimental animals. African Health Sciences. 2008;8(2).

Santhosh NS, Sinha S, Satishchandra P. Epilepsy: Indian perspective. Annals of Indian Academy of Neurology. 2014;17(Suppl 1):S3.

Holmes GL, Noebels JL. The epilepsy spectrum: targeting future research challenges. Cold Spring Harbor perspectives in medicine. 2016;6(7):a028043.

Holmes GL. Critical issues in the treatment of epilepsy. American Journal of Hospital Pharmacy. 1993;50(12_Suppl):S5-16.

Devinsky O. Cognitive and behavioral effects of antiepileptic drugs. Epilepsia. 1995;36:S46-65.

Mattson RH. Efficacy and adverse effects of established and new antiepileptic drugs. Epilepsia. 1995;36:S13-26.

Roger JP, Brian SM. Antiepileptic drugs in katzung, B, G. ed. Basic and clinical pharmacology. 7th edition Stanford: Appleton and lange. 1998:386-408.

Wahab A. Difficulties in treatment and management of epilepsy and challenges in new drug development. Pharmaceuticals. 2010;3(7):2090-110.

Scott RA, Lhatoo SD, Sander JW. The treatment of epilepsy in developing countries: where do we go from here? Bulletin of the World Health Organization. 2001;79:344-51.

Amoateng P, Woode E, Kombian SB. Anticonvulsant and related neuro-pharmacological effects of the whole plant extract of Synedrella nodiflora (L.) Gaertn (Asteraceae). Journal of Pharmacy, andBioallied Sciences. 2012;4(2):140.

Kosalge SB, Fursule RA. Investigation of ethnomedicinal claims of some plants used by tribals of Satpuda Hills in India. Journalof Ethnopharmacology. 2009;121(3):456-61.

Yineger H, Kelbessa E, Bekele T, Lulekal E. Plants used in traditional management of human ailments at Bale Mountains National Park, Southeastern Ethiopia. Journal of Medicinal Plants Research. 2013;2(6):132-53.

Deressa T, Mekonnen Y, Animut A. In Vivo anti-malarial activities of Clerodendrum myricoides, Dodonea angustifolia and Aloe debrana against Plasmodium berghei. Ethiopian Journal of Health Development. 2010;24(1).

Maina EN, Njau VN, Gavamukulya Y. Phytochemical analysis and anti-leishmanial activity of Clerodendrum myricoides and Salvadora persica Plant extracts against leishmania major. Journal of Complementary and Alternative Medical Research. 2020;29-44.

Buschmann J. The OECD guidelines for the testing of chemicals and pesticides. In Teratogenicity Testing. Humana Press, Totowa, NJ. 2013;37-56.

De Sarro A, Cecchetti V, Fravolini V, Naccari F, Tabarrini O, De Sarro G. Effects of novel 6-desfluoroquinolones and classic quinolones on pentylenetetrazole-induced seizures in mice. Antimicrobial agents and chemotherapy. 1999;43(7):1729-36.

Ojewole JA. Analgesic and anticonvulsant properties of Tetrapleura tetraptera (Taub)(Fabaceae) fruit aqueous extract in mice. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 2005;19(12):1023-9.

Tamboli AM, Rub RA, Ghosh P, Bodhankar SL. Antiepileptic activity of lobeline isolated from the leaf of Lobelia nicotianaefolia and its effect on brain GABA level in mice. Asian Pacific Journal of Tropical Biomedicine. 2012;2(7):537-42.

Hegde K, Thakker SP, Joshi AB, Shastry CS, Chandrashekhar KS. Anticonvulsant activity of Carissa carandas Linn. root extract in experimental mice. Tropical Journal of Pharmaceutical Research. 2009;8(2).

Son HL, Yen PT. Preliminary phytochemical screening, acute oral toxicity and anticonvulsant activity of the berries of Solanum nigrum Linn. Tropical Journal of Pharmaceutical Research. 2014;13(6):907-12.

Peng QL, Buz’Zard AR, Lau BH. Pycnogenol® protects neurons from amyloid-β peptide-induced apoptosis. Molecular Brain Research. 2002;104(1):55-65.

Choi J, Yoo Y, Park H. Inhibitory effects of the Korean red ginseng extract on the content of neurotransmitter-related components of the mouse brain in convulsion-induced model. Natural Product Sciences. 2007;13(4):384.

Löscher W, Schmidt D. Which animal models should be used in the search for new antiepileptic drugs? A proposal based on experimental and clinical considerations. Epilepsy research. 1988;2(3):145-81.

Upton N. Mechanisms of action of new antiepileptic drugs: rational design and serendipitous findings. Trends in pharmacological sciences. 1994;15(12):456-63.

Mante PK, Adongo DW, Woode E, Kukuia KK, Ameyaw EO. Anticonvulsant effect of Antiaris toxicaria (Pers.) Lesch.(Moraceae) aqueous extract in rodents. International Scholarly Research Notices; 2013.

Goel R, Saxena P. Pycnogenol protects against pentylenetetrazole-induced oxidative stress and seizures in mice. Current clinical pharmacology. 2019;14(1):68-75.

Sameh Mohamed Mostafa, EL-Nabtity, Ahmed Shaban Abdelaziz, Manar Salah Moselhi, Mario Giorgi. Anticonvulsant activity of hydro-alcoholic Phoenix dactylifera fruit extract and Pimpinella anisum Oil in Mice. American Journal of Animal and Veterinary Sciences.2019;14(2):127-138.

Rao VS, Rao A, Karanth KS. Anticonvulsant and neurotoxicity profile of Nardostachys jatamansi in rats. Journal of Ethnopharmacology. 2005;102(3):351-6.

Kouamé NM, Koffi C, N’Zoué KS, Yao NAR, Doukouré B, Kamagaté M. Comparative antidiabetic activity of aqueous, ethanol, and methanol leaf extracts of persea americana and their effectiveness in type 2 diabetic rats. Evidence-Based Complementary and Alternative Medicine. 2019;1–14.
DOI:10.1155/2019/5984570