Antimicrobial Evaluation of Plant Parts of Rauwolfia Vomitoria

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Stephen Chijioke Emencheta
Bessie Ifeoma Enweani
Angus Nnamdi Oli
Emmanuel Chinedum Ibezim
Ijeoma Eucharia Olaedo Imanyikwa


Aims: This study was aimed at evaluating the antimicrobial activities of fractions of Rauwolfia vomitoria against some isolates including; Salmonella typhi, Escherichia coli, Candida albicans, Aspergillus niger, Microsporon canis, and Trichophyton rubrum.

Methodology: Primary extraction was done on the dried and pulverized plant samples (leaves, barks, and root) using methanol, after which the crude extracts was fractionated using butanol, ethyl acetate, and n-hexane respectively. The samples were duly labelled according to the plant parts and solvents used. Agar diffusion and dilution methods on Mueller-Hinton agar (MHA) and Sabouraud dextrose agar (SDA) where used to determine the minimum inhibitory concentrations (MIC) and minimum bactericidal and fungicidal concentrations (MBC/MFC) respectively. The percentage inhibition diameter growth (PIDG) of the extracts and fractions were also determined.

Results: From the results, eleven (11) samples showed inhibitory activity on one or more of the test isolates. Extended spectrum activity was observed with five (5) of the samples, including; RVL ethyl acetate, RVL n-hexane, RVB crude, RVR crude, and RVR ethyl acetate against S. typhi, E. coli, and M. canis. Antifungal activity was observed only against M. canis. The ethyl acetate fraction of the leaves (RVLE) and crude methanol extract of the bark (RVBC) gave the least MIC both against M. canis at a concentration of 25 mg/ml, while the ethyl acetate fraction of the leaves (RVLE) at 12.5 mg/ml also against M. canis gave the least MBC/MFC. Using the inhibition zone diameters (IZD), the obtained PIDG showed apparently that the ethyl acetate fraction of the leaves (RVL ethyl acetate) was the most active against S. typhi (28.57%) and E. coli (20%).

Conclusion: The study lends support to the traditional use of the plant especially in treating gastro intestinal tract and skin infections.

Rauwolfia vomitoria, antimicrobial, methanol, n-hexane, ethyl acetate, butanol.

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How to Cite
Emencheta, S. C., Enweani, B. I., Oli, A. N., Ibezim, E. C., & Imanyikwa, I. E. O. (2020). Antimicrobial Evaluation of Plant Parts of Rauwolfia Vomitoria. Journal of Complementary and Alternative Medical Research, 12(1), 11-20.
Original Research Article


Ekeanyanwu CR. Traditional medicine in Nigeria: Current status and the future. Research Journal of Pharmacology. 2011;5(6):90–94. DOI:

Emencheta SC, Enweani IB, Oli AN, Okezie UM, Attama AA. Evaluation of antimicrobial activities of fractions of plant parts of Pterocarpus santalinoides. Biotechnology Journal International. 2019;23(3):1-11. DOI:

Oli AN, Obaji M, Enweani IB. Combinations of Alchornea cordifolia, Cassytha filiformis and Pterocarpus santalinoides in diarrhoegenic bacterial infections. BMC Research Notes. 2019;12:649. DOI:

Okwu GI, Enweani IB, Amengialue OO, Obiazi HAK. Comparative study of the phytochemical and antibacterial properties of two different varieties of Dioscorea bulbifera tubers. American Journal of Research Communication. 2016;4(7).

Okwuosa OMTB, Chukwura EI, Chukwuma GO, Okwuosa CN, Enweani IB, Agbakoba NR, Chukwuma CM, Manafa PO, Umedum CU. Phytochemical and antifungal activities of Uvaria chamae leaves and roots, Spondias mombin leaves and bark and Combretum racemosum leaves. African Journal of Medicine and Medical Sciences. 2012;41:99–103.

Orwa C, Mutua A, Kindt R, Jamnadass R, Simmons A. Agroforestree Database: A tree reference and selection guide version 4.0; 2009. Available:

Olatokunboh A, Kayode YO, Adeola OK. Anticonvulsant activity of Rauwolfia vomitoria (Afzel). African Journal of Pharmacy and Pharmacology. 2009;3(6): 319–322.

Kutalek R, Prinz A. Handbook of medicinal plants: African medicinal plants in Yaniv Z and U. Bachrach (Eds) New Delhi, CBS Publishers; 2007.

Lobay D. Rauwolfia in the treatment of hypertension. Integrative Medicine (Encinitas, Calif.). 2015;14(3):40–46.

Javaroni V, Neves MF. Erectile dysfunction and hypertension: Impact on cardiovascular risk and treatment. International Journal of Hypertension, 2012;627278. DOI:

Odugbemi T. A textbook of medicinal plants from Nigeria: Lagos, University of Lagos Press; 2008.

Usunobun U. Review manuscript: A review of some African medicinal plants. International Journal of Pharmaceutical and Biological Sciences. 2012;3(4):1–11.

Ijeh I, Omodamiro O, Nwana I. Antimicrobial effects of aqueous and ethanolic fractions of two species, Ocimum gratissimim and Xylopia aethiopica. African Journal of Biotechnology. 2005;4(9):53–956.

Waltrich KK, Hoscheid J, Prochnau IS. Antimicrobial activity of crude extracts and fractions of Vernonia polyanthes Less (assa-peixe) flowers. Revista Brasileira de Plantas Medicinais. 2015;17(4, Suppl. 2): 909–914. DOI:

Jayshree D, Dhruva J. Antidermatophytic activity of some medicinal plants, phytochemical analysis and isolation of active fractions from Piper longum. Journal of Pure and Applied Microbiology. 2013;7(4):3207–3312.

Okore V. A standard laboratory technique in pharmaceutics and pharmaceutical microbiology (2nd Edition). Naphtali prints, Nigeria. 2004;6–24.

Ogwuche C, Ayobo P, Odeja K. Antimicrobial properties and chemical compositions of petroleum ether extract of the aerial parts of Rauwolfia vomitoria obtained from Agbarho community of Delta State. Scientia Africana. 2016;15:2.

Ogwuche C, Adeyemi O. GC-MS analysis and antimicrobial studies of the methanol extract of aerial parts of Rauwolfia vomitoria obtain from Agbarho, Delta State, NISEB Journal. 2016;16(1).

Oseni LA, Ajisope BMU, Sunwiale SS. In-vitro bacteria growth inhibition potential of crude extracts of different parts of Rauwolfia vomitoria against five standard strains of selected human pathogenic microbes. World Journal of Pharmacy and Pharmaceutical Sciences. 2014;3(6):152-164.

Amole O, Yemitan O, Oshikoya K. Anticonvulsant activity of Rauwolfia vomitoria (Afzel). African Journal of Pharmacy and Pharmacology. 2009;3(6): 319-322.

Othman L, Sleiman A, Abdel-Massih RM. Antimicrobial activity of polyphenols and alkaloids in Middle Eastern plants. Frontiers in Microbiology. 2019;10:911. DOI:

Borokini T, Omotayo F. Phytochemical and ethnobotanical study of some selected medicinal plants from Nigeria. Journal of Medicinal Plants Research. 2012;6(7): 1106–1118,1123. DOI:

Nwogu LA, Igwe CU, Emejulu AA. Effects of Landolphia owerriensis Leaves extract on the liver function profile and haemoglobin concentration of albino rats. African Journal of Biotechnology. 2008;2(12):240-242.

Bele AA, Jadhav VM, Kadam VJ. Potential of tannins: A review. Asian Journal of Plant Sciences. 2010;9:209–214. DOI:

Coleman JJ, Ghosh S, Okoli I, Mylonakis E. Antifungal activity of microbial secondary metabolites. PloS one. 2011;6(9):e25321. DOI:

Onwuliri F, Wonang D. Biochemical and antimicrobial studies of Zingiber officinale and Allium sativum on the selected micro-organisms. B.Sc. Project in the department of Botany, University of Jos. 2003;59.