Subacute Toxicity and Hepatoprotective Effects of Sarcocephalus latifolius in Alloxan Induced Diabetic Rats
Journal of Complementary and Alternative Medical Research,
Various studies suggest that mortality due to liver disease in diabetic patients is very high; however, the recognition of DM as the primary cause of chronic liver disease is neglected in medical practice, we therefore evaluated the activities of Sarcocephalus latifolius leaf powder on the liver function of alloxan – induced diabetic rats. Forty-five healthy female albino rats were randomly assigned into 9 different groups; diabetes was induced intraperitonealy with 160 mg/kg of alloxan. Normal and diabetic rats were administered orally with 300, 600, 750 mg/kg/ b.w of S. latifolius. After 28 days, the animals were sacrificed for biochemical and histological studies.
The body weight of the normal and diabetic rats increased significantly with S. latifolius treatment, the increase observed in the blood glucose was brought down upon treatment with S. latifolius leaf powder. The activity of ALT increased significantly with 750 mg/kg of S. latifolius leaf powder, while low dose of the plant decreased it significantly in diabetic rats. GGT activity only decreased in the diabetic rats treated with 300 mg/kg of S. latifolius whereas albumin increased significantly (p<0.05) in all the groups administered S. latifolius powder relative to the untreated diabetic group. Bilirubin concentration only increased significantly (p<0.05) in the group administered 750 mg/kg of S. latifolius leaf powder. Histological changes including infiltration of the sinusoids and focal area by inflammatory cells and mild portal congestion were observed in all the groups except the normal and diabetic rats treated with 300 mg/kg of S. latifolius leaf powder. The result of the study showed that S. latifolius could only be encouraged for diabetes management only at low dose and might be hepatotoxic at high dose.
- Subacute toxicity
- Sarcocephalus latifolius
How to Cite
Saeedi P, Petersohn I, Salpea P, Malanda B, Karuranga S, Unwin N, Colagiuri S, Guariguata L, Motala AA, Ogurtsova K, Shaw JE, Bright D, Williams R. Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: Results from the International Diabetes Federation Diabetes Atlas, 9th edition. Diabetes Research and Clinical Practice. 2019;157:107843.
World Health Organization. Diabetes fact sheet N. 312, January 2015. Geneva: WHO; 2015. Available:www.who.int/mediacentre/factsheets/fs312/en/
Ganesan K, Rana MBM, Sultan S. Oral hypoglycemic medications. [Updated 2021 May 15]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2021. Available:https://www.ncbi.nlm.nih.gov/books/NBK482386/
Ezekwesili-Ofili JO, Okaka ANC. Herbal medicines in african traditional medicine, herbal medicine, Philip F. Builders, IntechOpen; 2019. DOI: 10.5772/intechopen.80348. Available:https://www.intechopen.com/chapters/64851
Abbiw DK. Useful plants of Ghana, intermediate technology publications and the royal botanic gardens kew, London. 1990;154-157.
Asase A, Oteng-Yeboah AA, Odamtten GT, Simmondsb MSJ. Ethnobotanical study of some Ghanaian anti-malarial plants. J Ethnopharmacol. 2005;99:273–279.
Asase A, Mensah GO. Traditional antimalaria phytotherapy remedies in herbal markets in Southern Ghana. J Ethnopharmacol. 2009;126(3):492–499.
Abbah J, Amos S, Chindoc B, Ngazalc I, Vongtaue HO, Adzuc B, Faridad T, Odutolad AA, Wambebec C, Gamaniel KS. Pharmacological evidence favouring the use of Nauclea latifolia in malaria ethnopharmacy: Effects against nociception, inflammation, and pyrexia in rats and mice. J Ethnopharmacol. 2010;127:85–90.
Nadia BMA, Emmanuel AM, Ernest ZN, koffi K. Phytochemical study, acute toxicity, and fertility potential effect of S. latifolius (Smith) on the histology of wistar rats testicles. European Journal of Medicinal Plants. 2021;32(2):62-69.
Reitman S, Frankel SA. Colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American Journal of Clinical Pathology. 1957;28(1):56.
Sherlock S. P. 204 in Liver Disease, Churchill, London; 1951.
Lucchesi AN, Cassettari LN, Spadella CT. Alloxan-induced diabetes causes morphological and ultrastructural changes in rat liver that resemble the natural history of chronic fatty liver disease in humans. J Diabetes Res; 2015.
Romagnoli M, Gomez-Cabrera MC, Perrelli MG, Biasi F, Pallardó FV, Sastre J, Poli G, Viña J. Xanthine oxidase-induced oxidative stress causes activation of NF-kappaB and inflammation in the liver of type I diabetic rats. Free Radic Biol Med. 2010;49:171–7.
Lala V, Goyal A, Bansal P, Minter DA. Liver function tests. [Updated 2021 May 9]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2021. Available:https://www.ncbi.nlm.nih.gov/books/NBK482489/
Orwa C, Mutua A, Kindt R, Jamnadass R, Simona A. Agroforestree database: A tree reference and selection guide version 4.0; 2009. Available:http/www.worlddagroforestry.org/af/treed bi Accessed on 10/6/2019.
Magili ST, Maina HM, Barminas JT, Toma I. Toxicity study of aqueous leaf extracts of S. latifolius (Rubiaceae) in rats. Merit Research Journal of Environmental Science and Toxicology. 2014;2(6):120-128.
Abstract View: 92 times
PDF Download: 38 times