Biochemical Constituents and Anticancer Activities of the Essential Oils of Lannea egregia Engl. & K. Krause

Oluwatosin T. Ayodele

Department of Chemistry, University of Ibadan, Ibadan-200284, Nigeria.

Olaoluwa O. Olaoluwa *

Department of Chemistry, University of Ibadan, Ibadan-200284, Nigeria.

Olapeju O. Aiyelaagbe

Department of Chemistry, University of Ibadan, Ibadan-200284, Nigeria.

Flamini Guido

Dipartmento di Farmacia, Via Bonanno 6, Universita di Pisa, Pisa-56126, Italy.

Omonike O. Ogbole

Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria.

Toluwanimi E. Akinleye

Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria.

Adekunle J. Adeniji

Department of Virology, University of Ibadan, Ibadan, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

Introduction: The Increase in cancer deaths has raised concerns among scientists to research on prospective anticancer agents. Lannea egregia was selected for this research because it has been identified to have ethnobotanical claims in relation to the cure of cancer in South-West Nigeria.

Aims: To analyze the chemical constituents and determine the anticancer properties of the essential oils obtained from Lannea egregia Engl. & K. Krause Leaf, twig and stem-bark.

Study Design: This study was designed to assay the chemical constituents of Lannea egregia and based on the ethnobotanical claims, the anticancer activities of this plant was studied.

Place and Duration of Study: Department of Chemistry, Dipartmento di Farmacia, Via Bonanno 6, Universita di Pisa and University of Ibadan and Department of Virology, University of Ibadan, Ibadan, Nigeria. July 2018- April 2021.

Methodology: Essential oils (EOs) from leaf, twig and stem-bark of Lannea egregia were extracted by hydro-distillation. Chemical compositions were characterized using Gas Chromatography–Mass Spectrometry (GC–MS). Cytotoxicity screenings of EOs were determined on human rhabdomyosarcoma (RD) and breast adenocarcinoma (MCF-7), using MTT [3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] cell viability assay.

Results: Leaf, twig and stem-bark EOs gave good yields of 0.12%, 0.43% and 0.33%, respectively.

Fifty-five, thirty-three and thirty-eight compounds were identified in leaf, twig and stem-bark EOs, accounting for 99.1%, 97.8% and 95.8% of them. Most abundant compound in leaf, stem-bark and twig EOs was β-caryophellene (28.2, 23.4 and 13.2%, respectively).

Leaf EO displayed strong cytotoxicity against RD and MCF-7, with 50% cytotoxicity (CC50) of 1.69 and 15.81µg/ml, respectively. Twig EO exhibited good selectivity with selectivity index of 24.8 and 10.8 for RD and MCF-7, respectively.

Conclusion: Results showed that EOs have good anticancer properties.

Keywords: Lannea egregia, essential oils, GC-MS, sesquiterpenes, anticancer activity


How to Cite

Ayodele , Oluwatosin T., Olaoluwa O. Olaoluwa, Olapeju O. Aiyelaagbe, Flamini Guido, Omonike O. Ogbole, Toluwanimi E. Akinleye, and Adekunle J. Adeniji. 2023. “Biochemical Constituents and Anticancer Activities of the Essential Oils of Lannea Egregia Engl. & K. Krause”. Journal of Complementary and Alternative Medical Research 21 (4):33-42. https://doi.org/10.9734/jocamr/2023/v21i4446.

Downloads

Download data is not yet available.

References

Gavhane YN, Shete AS, Bhagat AK, Shinde VR, Bhong KK, Khairnar GA, Yadav, AV. Solid Tumors: Facts, challenges and solutions. IJPSR. 2011: 2(1):1-12.ISSN : 0975-9492

Sylla BS, Wild CP. A million Africans a year dying from cancer by 2030: what can cancer research and control offer to the continent? Int J Cancer. 2012;130(2): 245-250.

Available: https://doi.org/10.1002/ijc.26333.

Emami A, Sepheri Z, Gordon JW, Ghavami S. Biological and clinical aspects of Rhabdomyosarcoma. IJBSM. 2017;2(1): 1-4. DOI:10.15171/IJBSM.2017.01.

Siddiqa A, Long L, Li L, Marciniak R, Kazhdan I. Expression of HER-2 in MCF-7 breast cancer cells modulates anti-apoptotic proteins Survinin and Bci-2 via the extracellular signal-related kinase (ERK) and phosphoinositide-3 kinase (PI3K) signalling pathways. BMC Cancer. 2008: 8:129. DOI:10.1186/1471-2407-8-129

Yang L, Takimoto T, Fujimoto J. Prognostic model for predicting overall survival in children and adolescents with rhabdomyosarcoma. BMC Cancer. 2014; 14:654. DOI: 10.1186/1471-2407-14-654

Nehad A, Nouf S., Amal A. and Mehal A.Cytotoxic effects of some essential oils on Mcf-7, Hfs and Hct116 cell lines. AJB. 2020: 19(6):392-399. DOI: 10.5897/AJB2020.17160

Song Y, Sun H, Zhang A, Yan G, Han Y, Wang X. Plant-derived natural products as leads to anti-cancer drugs. JMPHTR. 2014: 2:6-15. ISSN 2053-1826

Adegoke OA, Ayodele OT. Novel manganese colorimetric chemosensing investigations of Indigofera macrophylla Schum (Thonn.) stem and leaf dye extracts. Nig. J. Pharm. Res. 2017;13(2):115-125.

Asakawa Y, Ludwiczuk A, Nagashima F. Chemical Constituents of Bryophytes: Bio and chemical diversity, biological activity, and chemosystematics. New York, NY, USA; Springer. 2012:95.

E-ISSN 2192-4309.

Dhifi W, Bellili S, Jazi S, Bahloul N, Mnif W. Essential Oils’ chemical characterization and investigation of some biological activities: A Critical Review: Medicines. 2016: 1-2. DOI: 10.3390/medicines3040025.

Idowu P, Ekemezie L, Olaiya C. Phytochemical, antioxidant and antimicrobial studies of Lannea egregia Engl & K. Krause (Anacardiaceae) extracts and chromatographic extracts. AJOL. 2020;19(1):348-363. DOI:10.4314/jopat.v19i1.4

Rahman M, Khatun A, Uddin SJ, Shilpi JA. Comparative effect of lannea coromandelica (houtt.) Merr. Leaves and stem barks on acetic acid induced pain model in mice and chromogenic reagents: exploring the analgesic potential and phytochemical groups. Pharmacologyonline. 2016;1:146-152. ISSN: 1827-8620

Islam M and Tahara S. Dihydroflavonols from Lannea coromandelica. Phytochemistry. 2000.54(8):901-907.

Available: doi.org/10.1016/S0031-9422(00)00048-0

Bharti M, Kanchan K, Saifu R. review of medicinal uses, phytochemistry, pharmacological properties, extraction methods and toxicology of Lannea microcarpa (African Grapes). Bentham Science. 2021;7(1):125-137. DOI: 10.2174/2215083805666190626095609

Kadiri M, Ojewumi AW, Onatade TN. Indigenous uses and phytochemical contents of plants used in the treatment of menstrual disorders and after- child birth problems in Abeokuta South Local Government Area of Ogun State, Nigeria. JDDT. 2015: 5(3):33-42. ISSN: 2250-1177.

Idu M, Joseph OE, Harriet ME. Documentation on medicinal plants sold in markets in Abeokuta, Nigeria. Trop. J. Pharm Res. 2010: 9(2):110-118.

Soladoye MO, Amusa NA, Raji-Esan SO, Chukwuma EC, Taiwo AA. Ethnobotanical Survey of Anti-Cancer Plants in Ogun State, Nigeria. J. Biol. Sci. 2010: 1(4):261-273.

Rafiu B, Sonibare A, Adesanya E. Phytochemical screening, antimicrobial and antioxidant studies of Lannea egregia Engl. and K. Krause (Anacardiaceae) stem bark. JOMPED.2019;3(1):a62. DOI: https://doi.org/10.4102/jomped.v3i1.62.

Olaoluwa OO, Oladosu IA, Aiyelaagbe OO, Flamini G. Essential oils of aerial parts of Crassocephalum rubens (Juss. ex jacq,), S. moore and Cardiosperumum grandiflorum (sweet) Stem. J Altern Complement Med. 2018: 1-8.

DOI:10.9734/JOCAMR/2018/42485

Bayala B, Bassole I, Scifo R, Gnuola C, Morel L, Lobaccaro J and Simpore J. Anticancer activities of essential oil and their chemical constituents Am. J. Cancer Res. 2014. 4: 591-607

Ogbole OO, Segun PA, Adeniji AJ. In-vitro cytotoxic activity of medicinal plants from Nigeria ethnomedicine on Rhabdomyosarcoma cancer cell line and HPLC analysis of active extracts. BMC Complement.med. 2017;17:494. DOI:10.1186/s12906-017-2005-8

Ogundajo A, Ewekeye T, Sharaibi O, Owolabi M, Dosoky N, Setzer W. Antimicrobial activities of sesquiterpene-rich essential oils of two medicinal plants, Lannea egregia and Emilia sonchifolia, from Nigeria. MDPI. 2021; 10:488. DOI: 10.3390/plants10030488.

Dahham S, Tabana Y, Iqbal M, Ahamed M, Ezzat M, Majid A, Majid A. The anticancer, antioxidant and antimicrobial properties of the sesquiterpene ß-caryophellene from essential oil of Aquilaria crassna. Molecules. 2015;20:11808-11829. DOI: 10.3390/molecules200711808

UKEssays. 2018. Chemotherapy and Selective Toxicity. Available:https://www.ukessays.com/essays/biology/chemotherapy-and-selective-toxicity-biology-essay.php?vref=1