Anti-Inflammatory Activity of Ethanol Leaf Extract of Arthropteris Orientalis in Wistar Albino Rat
Akhigbe Faith Omoighele
Department of Pharmacology and Therapeutics, University of Nigeria, Enugu, Nigeria.
Ckikere A. Anusiem
Department of Pharmacology and Therapeutics, University of Nigeria, Enugu, Nigeria.
Samuel I. Ghasi
Department of Pharmacology and Therapeutics, University of Nigeria, Enugu, Nigeria.
Ajirioghene E. Akpotu *
Department of Pharmacology, PAMO University of Medical Sciences, Port-Harcourt, Rivers State, Nigeria.
Chidi E. Ezerioha
Department of Pharmacology, University of Port Harcourt Rivers State, Nigeria.
Funso-Babarimisa, Funso
Department of Pharmacology Kogi State University, Anyigba, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
The study investigated the anti-inflammatory activity of ethanol leaf extract of Arthropteris orientalis in wistar albino rat. The plant extract was obtained with the procedure as described by Khalifa et al. [1] and both acute and chronic anti-inflammatory properties was assessed. Five groups of five animals each were utilized for acute and chronic inflammation respectively. Anti-inflammatory effect of the extract was evaluated on egg albumin induced paw edema in albino rats and edema induced by formalin in the wistar albino rats’ paw for acute and chronic inflammation respectively. The extract had 26.665% tannin while flavonoid, saponin, phenol, and glycoside had 0.784%, 0.631%, 0.183% and 0.057% respectively. The result revealed that the extract at 500 mg/kg exhibited significant inhibition (p<0.05) of systemic acute paw edema at the 4th hour of the second phase of edema in comparison with the standard drug. The results reveal that intraperitoneal administration of ethanol leaf extract of Arthropteris orientalis on formalin-Induced paw edema in wistar albino rats at (100 and 500mg/kg) and (100mg/kg) exhibited significant inhibition (p<0.05) in mean paw volumes on day 1 and 2 respectively. The extract did not produce any significant hematological change. These results show that Arthropteris orientalis has potential anti-inflammatory properties.
Keywords: Anti-inflammatory, Arthropteris orientalis, Formalin, Hematology
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References
Khalifa NM, Al-Omar MA, Ali OM. Synthesis and reactions of some new (E)-2-сyano-N′-[1-(pyren-3-yl)ethylidene]acetohydrazide derivatives. Russian Journal of General Chemistry. 2017;87(9):2083–2086. Available:doi.org/10.1134/s1070363217090250
Winiger Fabian. The spirituality of others and the who discourse on traditional medicine; 2022 Available:doi.org/10.1093/oso/9780192865502.003.0005
Jan Rahmatullah , Sajjad Asaf, Muhammad Numan Lubna, Kyung-Min Kim. Plant secondary metabolite biosynthesis and transcriptional regulation in response to biotic and abiotic stress conditions. Agronomy; 2021.
Available:doi.org/10.3390/agronomy11050968
Addo-Fordjour P, Yeboah-Gyan K, Lawson BWL, Akanwariwiak WG. Diversity and distribution of ferns on the campus of the Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana. Journal of the University of Science and Technology. 2007;27(1) Available:https://doi.org/10.4314/just.v27i1.33022
Petkov Venelin, Iva Slavova, Desislava Teneva, Tzvetelina Mladenova, Plamen Stoyanov, Mariana Argirova. Phytochemical study and biological activity of three fern species of the asplenium genus growing in Bulgaria. The Natural Products Journal; 2021 DOI: 10.2174/2210315511666210512024716
Chen L, Deng H, Cui H, Fang J, Zuo Z, Deng J. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget. Impact Journals, LLC. 2018;9(6):7204–7218 Available:https://doi.org/10.18632/oncotarget.23208.PMC5805548
Ferrero-Miliani L, Nielsen OH, Andersen PS, Girardin SE. Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1beta generation. Clinical and Experimental Immunology. 2007;147(2):227–235 Available:doi.org/10.1111/j.1365-2249.2006.03261.x
Sriuttha P, Sirichanchuen B, Permsuwan U. Hepatotoxicity of nonsteroidal anti-inflammatory drugs: A systematic review of randomized controlled trials. Int J Hepatol. 2018;5253623.
Okaiyeto Kunle, Oluwafemi O. Oguntibeju. African herbal medicines: Adverse effects and cytotoxic potentials with different therapeutic applications. Int. J. Environ. Res. Public Health; 2021 Available:doi.org/10.3390/ijerph18115988
Ajirioghene AE, Ani CO, Ajebor DN, Elavieniso AF,Okolo KO. Methanolic leaf extract of Dryopteris dilatata reverses kidney injury on streptozotocin-induced diabetic male Wistar rats. Niger J Exp Clin Biosci. 2022;10:65-73.
Ajirioghene E, Akpotu Ani C, Nworgu C, Pamela O, Igwe U, Uzoigwe J, Adeyemo M, Nwaeme O, Onwujekwe O. Antidiabetic and Anti- hyperlipidemic effects of ethanolic extract of Dryopteris dilatata leaves. Journal of Diabetes and Endocrinology. 2018;9(3):20-27.
Prabhavathi RM, Prasad MP, Jayaramu. Studies on qualitative and quantitative phytochemical analysis of Cissus quadrangularis Adv. Adv. Appl. Sci. Res. 2016;7:11–17.
Madhu M, Sailaja V, Satyadev T, Satyanarayana MV. Quantitative phytochemical analysis of selected medicinal plant species by using various organic solvents. Journal of Pharmacognosy and Phytochemistry. 2016;5(2):25-29.
Lorke D. A new approach to practical acute toxicity. Archive Toxicol. 1983;53:275-289.
Shabbir A, Batool SA, Basheer MI, Shahzad M, Sultana K, Tareen RB, et al. Ziziphora clinopodioides ameliorated rheumatoid arthritis and inflammatory paw edema in different models of acute and chronic inflammation. Biomed. Pharmacother. 2018;97:1710–1721 Available:doi:10.1016/j.biopha.2017.11.118
Oyeleke SA, Ajayi AM, Umukoro S, Aderibigbe A, Ademowo OG. Anti-inflammatory activity of Theobroma cacao L. stem bark ethanol extract and its fractions in experimental models. J. Ethnopharmacol. 2018;222:239–248. DOI: 10.1016/j.jep.2018.04.050
Owolabi OO, James DB, Sani I, Andongma BT, Fasanya OO, Kure B. Phytochemical analysis, antioxidant and anti-inflammatory potential of feretia apodanthera root bark extracts. BMC Complementary and Alternative Medicine. 2018;18(1). Available:doi.org/10.1186/s12906-017-2070-z
Hamalainen M, Nieminen R, Vuorela P, Heinonen M, Moilanen E. Anti-Inflammatory effects of flavonoids: Genistein, Kaempferol, Quercetin, and Daidzein inhibit stat-1 and NF-κB activations,Whereas flavone, isorhamnetin, naringenin, and pelargonidin inhibit only NF-κB Activation along with Their Inhibitory Effect on iNOS Expression and NO Production in Activated Macrophages. Mediat Inflamm. 2007;1–10.
Altemimi Ammar, Naoufal Lakhssassi, Azam Baharlouei, Dennis G Watson, David A. Lightfoot phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants (Basel); 2017. DOI: 10.3390/plants6040042
Ambreen M, Mirza SA. Evaluation of anti-inflammatory and wound healing potential of tannins isolated from leaf callus cultures of Achyranthes aspera and Ocimum basilicum. Pakistan Journal of Pharmaceutical Sciences. 2020;33(1):361–369.
Kehinde P, Olamilosoye Rufus O, Akomolafe Olumide S, Akinsomisoye A, Modinat A, Adefisayo Quadri K Alabi. The aqueous extract of Ocimum gratissimum leaves ameliorates acetic acid-induced colitis via improving antioxidant status and hematological parameters in male Wistar rats. Egyptian Journal of Basic and Applied Sciences; 2023 Available:doi.org/10.1016/.
Omodamiro O, Jimoh M. A Evaluation of anti-inflammatory and diuretic effects of ethanol leaf extract of piper guineense on wistar albino rats. American Journal of Ethnomedicine. 2014;1(4):250–259.
Barung Elisabeth Natalia, Jovie Mien Dumanauw, Meilany Feronika Duri, Donald Emilio Kalonio. Egg white-induced inflammation models: A study of edema profile and histological change of rat's paw. J Adv Pharm Technol; 2021. DOI: 10.4103
Duke JA. Handbook of Biologically active phytochemicals and their activities. CRC Press; 1992.
Matotoka M, Mashilo Gabriel T Mashabela, Peter Masoko. Phytochemical content, antibacterial activity, and antioxidant, anti-inflammatory, and cytotoxic. Effects of traditional medicinal plants against respiratory tract bacterial pathogens. Evid Based Complement Alternat Med; 2023. DOI: 10.1155/2023/1243438
Fu KY, Light AR, Maixner W. Long-lasting inflammation and long-term hyperalgesia after subcutaneous formalin injection into the rat hindpaw. The Journal of Pain: Official Journal of the American Pain Society. 2001;2(1):2– 11. Available:doi.org/10.1054/jpai.2001.9804
Tong Zhenkai, Wenfeng He, Xiao Fan, Aiwei Guo. Biological function of plant tannin and its application in animal health. Front Vet Sci; 2021 DOI: 10.3389/fvets.2021.803657
Wun Y, Zhou C, Li X, Song L, Wu X, Lin W, Chen H, Bai H, Zhao J, Zhang R, Sun H, Zhao Y. Evaluation of antiinflammatory activity of the total flavonoids of laggera pterodonta on acute and chronic inflammation models. Phytotherapy Research: PTR. 2006;20(7):585–590 Available:doi.org/10.1002/ptr.1918
Sarkar Chandan, Milon Mondal, Bilkis Khanom, Md. Monir Hossain, Md. Solayman Hossain, Antoni Sureda, Muhammad Torequl Islam, Miquel Martorell, Manoj Kumar, Javad Sharifi-Rad, Ahmed Al-Harrasi, and Ahmed Al-Rawahi. Heliotropium indicum L.: From farm to a source of bioactive compounds with therapeutic activity evid based complement alternat med; 2021. DOI: 10.1155/2021/9965481
Betancourt-Alonso MA, Orihuela A, Aguirre V, Vázquez R, Flores-Pérez FI. Changes in behavioural and physiological parameters associated with Taenia pisiformis infection in rabbits (Oryctolagus cuniculus) that may improve early detection of sick rabbits. World Rabbit Science. 2011;19(1) Available:https://doi.org/10.4995/wrs.2011.801
Nana William Yousseu, Justin Rodrigue Billong Mimb, Albert Donatien Atsamo,Eric Gonzal Tsafack, Stephanie Flore Djuichou Nguemnang, Zenab Linda Fagni Njoya, Vanessa Mba Matah Marthe, Yacine Karelle Madjo Kouam, Marius Mbiantcha,and Gilbert Ateufack. In vitro and in vivo anti-inflammatory properties of the hydroethanolic extract of roots of vennonia guineensis (Asteraceae). International Journal of Inflammation; 2023 Available:doi.org/10.1155/2023/7915367
Asakawa Y. Recent advances in phytochemistry of bryophytesacetogenins, terpenoids and bis(bibenzyl)s from selected Japanese, Taiwanese, New Zealand, Argentinean and European liverworts. Phytochemistry. 2001;56:297–312.