Journal of Complementary and Alternative Medical Research https://journaljocamr.com/index.php/JOCAMR <p style="text-align: justify;"><strong>Journal of Complementary and Alternative Medical Research (ISSN: 2456-6276)</strong> aims to publish high quality papers (<a href="/index.php/JOCAMR/general-guideline-for-authors">Click here for Types of paper</a>) in the areas of Complementary, Alternative and Integrative medical research. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.</p> en-US contact@journaljocamr.com (Journal of Complementary and Alternative Medical Research) contact@journaljocamr.com (Journal of Complementary and Alternative Medical Research) Thu, 22 Oct 2020 12:04:16 +0000 OJS 3.1.1.4 http://blogs.law.harvard.edu/tech/rss 60 Antioxidant activities of Dichrostachys cinerea (L.) Wight et Arn (Leguminosae): Correlations between the Polyphenol Level and the Antioxidant Activity https://journaljocamr.com/index.php/JOCAMR/article/view/30185 <p><strong>Aims: </strong>Free radicals are involved in many diseases. Antioxidants help control them. The overproduction of free radicals or reduction of natural antioxidants promotes the destruction of cells in our body, for example in neurodegenerative diseases. The aim of our study is to evaluate the correlation between the level of polyphenol and antioxidant activity of <em>Dichrostachys cinerea</em> barks he work was carried out at the phytochemistry department of the Institute of Pharmacopoeia and Traditional Medicine (Gabon).</p> <p><strong>Methodology:</strong> Different extracts of <em>Dichrostachys cinerea </em>barks were obtained with polarity solvent (dichloromethane, ethyl acetate, butanol, methanol and water).&nbsp; The antioxidant activity is evaluating using respectively FRAP method (Ferric Reducing Antioxidant Power), antiradical activity by the method of inhibition of DPPH radical (2.2-diphenyl-1-picrylhydrazyl), cations ABTS radical (2.2'-azinobis-[3-acid-6-sulfonic ethylenzothiazoline]).</p> <p><strong>Results:</strong> Ethyl acetate and dichloromethane extract have the higher value for polyphenolic compounds, respectively 52,27±0,66 mg EAG / g and 49,72±0,55 mg EAG / g. Aqueous extract have the lower value 20,67±1,05 mg EAG / g. The different fractions of <em>Dichrostachys cinerea</em> have antioxidant effect, and this effect is in correlation with its constituents: polyphenols, tannins and flavonoids. Results were compared to standard antioxidants such as ascorbic acid and quercetin.</p> <p><strong>Conclusion:</strong> This study confirms higher is the level of polyphenols greater is the antioxidant power.</p> Reine Raïssa Rolande Aworet Samseny, Line-Edwige Mengome, Sophie Aboughe Angone ##submission.copyrightStatement## https://journaljocamr.com/index.php/JOCAMR/article/view/30185 Thu, 22 Oct 2020 00:00:00 +0000 Comparative Evaluation of the Larvicidal Properties of Methanol Extracts and Fractions of Ocimum gratissimum L. and Ocimum basilicum L. Leaves (Lamiaceae) on the Fourth Instar Larvae of Culex quinquefasciatus L. and Control of Filariasis https://journaljocamr.com/index.php/JOCAMR/article/view/30187 <p><strong>Background:</strong> Botanical insecticides may serve as suitable alternatives to synthetic insecticides because they contain bioactive chemicals. They are relatively safe, biodegradable and readily available in many areas of the world.</p> <p><strong>Objective:</strong> The aim of this study is to comparatively evaluate the larvicidal properties of methanol extracts and fractions of <em>Ocimum gratissimum</em> and <em>Ocimum basilicum</em> leaves on fourth instar larvae of <em>Culex quinquefasciatus</em>, the vector of filariasis.</p> <p><strong>Methods:</strong> The plants were identified, collected, air-dried and pulverized. The powdered leaves were macerated in 100% redistilled methanol for 72 hours, filtered and concentrated to thick extracts. After the preliminary work was done as activity-guided process, the crude extracts were partitioned into different solvents and again concentrated to thick fractions. The percentage yields were calculated and recorded. The larvae were collected from a location at Ewet Housing Estate, Uyo, Akwa Ibom State. Toxicity was evaluated by exposing fourth instar larvae of <em>Culex quinquefasciatus</em> to a concentration range of 0.0625 to 1.000mg/mL of the methanol crude extracts and fractions. The larval mortalities were recorded after 24 hours of exposure and LC<sub>50</sub> values were determined using the non-linear regression analysis of a statistical package graph pad prism®.</p> <p><strong>Results:</strong> The results of percentage mortalities of methanol crude extracts ranged from 2.5±0.50 to 67.50±1.05% for <em>O. gratissimum</em> and 2.5±0.50 to 100±0.00% for <em>O. basilicum</em> with their LC<sub>50</sub> of 0.79mg/mL and 0.18mg/mL respectively.&nbsp; The solvent partitioned fractions also showed that the percentage mortalities of n-hexane and chloroform of <em>O. gratissimum</em> ranged from 5±1.00% to 100±0.00% and 17.5±1.5% to 97.5±0.5% with their LC<sub>50</sub> of 0.29mg/mL and 0.32 mg/mL respectively, while those of <em>O. basilicum</em> ranged from 0.25±0.5% to 100±0.00% for n-hexane and 7.5±0.5% to 92.5±1.5% for chloroform fractions with their LC<sub>50</sub> of 0.42mg/mL and 0.39 mg/mL compared to that of Nicotine (positive control) with LC<sub>50</sub> of 0.01mg/mL with a percentage mortality range of 10±1.00 to 100±0.00%.&nbsp;</p> <p><strong>Conclusion</strong>: <em>O. basilicum</em> leaf methanol crude extract was more than four times active than that of <em>O. gratissimum,</em> but n-hexane and chloroform solvent partitioned fractions of <em>O. gratissimum</em> were more active than those of <em>O. basilicum</em> considering their percentage mortalities and LC<sub>50</sub>. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Hence, both methanol crude extracts of <em>O. basilicum</em> and n-hexane fraction of <em>O. gratissimum</em> &nbsp;&nbsp;&nbsp;&nbsp;have greater potentials as larvicides which can also be used in the control of <em>C. quinquefasciatus</em> larvae.</p> Romanus A. Umoh, Imoh I. Johnny, Anwanabasi E. Udoh, Akwaowoh A. Elijah, Omodot T. Umoh, Lovina E. Essiet ##submission.copyrightStatement## https://journaljocamr.com/index.php/JOCAMR/article/view/30187 Mon, 26 Oct 2020 00:00:00 +0000 Application of Platelet Rich Fibrin, Platelet Rich Plasma, Plasma Rich Growth Factors in Different Fields of Dentistry: An Overview https://journaljocamr.com/index.php/JOCAMR/article/view/30186 <p>A huge number of studies have demonstrated that platelet-rich preparations applied to surgical sites, injuries, or wounds are effective way to promote soft tissue healing and bone growth. The potential use of&nbsp; platelet rich preparations like Platelet - rich plasma and Platelet - rich fibrin are new boon to dentistry termed under the general acronyms PRP (Platelet-Rich Plasma) or PRF (Platelet-Rich Fibrin), is an important current transversal field of research across many fundamental and clinical disciplines.The third important is PRGF.&nbsp; Plasma Rich in Growth Factor (or PRGF) is a type of plasma enriched of proteins and circulating growth factors able to aid the bone and soft tissue regeneration. PRGF includes plasma proteins and coagulative factors and is then more valuable compared to PRP. There are many scientific literature which cover one or other concise aspect of platelet rich preparation. This review aims at covering all basic definitions, key element, history, preparation, differences between PRF and PRP and applications in a simplified manner.</p> Geetanjali Singh, Bhawna Kumari, Harangad Singh Grover, Akriti Mahajan, Farhat Jabeen, Ajay Kumar ##submission.copyrightStatement## https://journaljocamr.com/index.php/JOCAMR/article/view/30186 Sat, 24 Oct 2020 00:00:00 +0000