Journal of Complementary and Alternative Medical Research

Warts are the results of human papillomavirus infection on the skin. Warts usually occur on the humans’ hands, feet, and also in other locations of the body. We report here a study of three warts cases treated with homeopathic medicines. Two medicines (Medorrhinum and Natrum muriaticum) combinedly were administered orally for treating all three patients. Within 3-14 weeks of treatment, warts disappeared relieving all the patients from suffering. The result of this study is encouraging because the warts were removed without harming the patients and in a cost-effective way. This study would help to develop awareness among medical practitioners regarding the available homeopathic treatment options for warts.

The auricular acupuncture is the therapeutic applied in the microsystem of outer ear. In this area, the local innervations stimulate the brain regions resulting in diagnostic and treatment system. There are three auricular acupuncture points used together as positive impulse to the therapy: Shen men (SHM), Kidney (K), Autonomic Nervous System (sympathetic) (ANS). Neurometry is the neuroscience tool that uses computing to observe, through images, the brain's response to external stimuli. Our aim is to identify the brain answers to the three auricular acupuncture points used together, SHM, K, ANS through the neurometry. It was observed three volunteers during four different moments: in the beginning, before the procedure; 15 minutes, 24 hours and 48 hours after the needles application. Central nervous system effects, physical health patterns, emotional exhaustion, anxiety control, blood flow, respiratory and cardio functional changes were recorded.

We conclude that the chosen points positively interfere in the control of anxiety, in the cardio-functional system, in the sympathetic nervous system and in parasympathetic functional oxygenation and that neurometry is qualified for further studies on the subject.

Aim: The aim of this study therefore was to evaluate the antidiabetic potentials of Morus mesozygia Linn. Stapf., Leaf extrcts in Streptozotocin-Induced Diabetic Rats.

Study Design:  The study is an experimental case-controlled study.

Place and Duration of Study: This study was carried out at the Biochemistry Research Laboratory, University of Port Harcourt, Rivers State, Nigeria, between June 2018-April 2019.

Methodology: A total of 65 male albino rats that weighed between 150g to 200g were used for this research study. Three different extracted solvents; aqueous, ethanolic and methanolic leaves extracts were administered to different groups of the rats. The male albino rats for this study were induced with a single dose of 40mg/kg b.wt, intraperitoneally of streptozotocin in 0.1M of citrate buffer, pH 4.5. The diabetic male rats were those whose fasting blood glucose (FBG) was from 250 mg/dl or 13 mmol/L and above.

Results: The results showed that there were significant decreases (p<0.05) in, FBG, increased insulin and increased C-peptide and increased HOMA-IR concentration of induced diabetic male rats treated orally with 400 mg/kg of MMLS., when compared with the values of the diabetic male rats in treated orally with 200 mg/kg of MMLS., and non-significant decrease(p<0.05) in FBG, HOMA-IR increased insulin, increased C-peptide concentrations in the diabetic male rats treated orally for 30 days with 400 mg/kg in dose of methanolic leaves extracts of MMLS., in the group treated with 400 mg/kg methanol, when compared to the concentration of diabetic male in treated orally for 30 days with 200 mg/kg of methanolic leaves extracts of MMLS. Compared likewise with that treated with 100mg/kg of metformin standard drug. There were also significant decrease (p<0.05) in FBG, HOMA-IR, C-peptide and increased insulin concentrations in diabetic male rats treated with 200 mg/kg in dose of ethanolic and methanolic leaves extracts of MMLS., when compared with the values in the diabetic male rats treated orally with 400 mg/kg in dose of ethanolic and methanolic leaves extracts of MMLS., compared with values of diabetic male rats treated orally with 100mg/kg in dose of metformin standard drugs compared with the controls.

Conclusion: From the findings of this study, we conclude that streptozotocin increased FBG levels, while the Methanolic, ethanolic and aqueous extracts of Morus mesozygia Linn. S improved FBG, C-peptide, insulin and HOMA levels in a dose-dependent manner, with the methanolic extract having the best ameliorative effect, probably due to its more phytochemical composition.

Aims: The aim of this study was to evaluate the anti-diabetic, hepatorenoprotective and antilipidemic potentials of Syzygium aromaticum (clove) in albino rats.

Study Design: This study is a non-randomized experimental study.

Place and Duration of Study: Department of Medical Laboratory Technology, University of Port Harcourt, Nigeria, between September, 2019 and December, 2019.

Methodology: Thirty-five male Wister rats with weight between 145 to 150 g were randomly selected into seven groups of five rats each. The first group served as Negative control (group 1). The second group was the positive control (Diabetic group). The remaining five groups being the treatment groups (3-7). Diabetes was induced intraperitoneally with 65 mg/kg of streptozotocin (STZ) single dose. Group 3 Diabetic group treated with metformin (100 mg/kg); Group 4 Diabetic group treated with low dose clove (250 mg/kg). Group 5 Diabetic group treated with low dose clove and metformin; Group 6 Diabetic group treated with high dose clove (750 mg/kg), while Group 7 were Diabetic group treated with high dose clove (750 mg/kg) and metformin for six weeks. Blood samples were collected via cardiac puncture in appropriate EDTA, heparinized and sterile bottles for standard laboratory investigations of lipid profile, glucose, liver enzymes, urea and creatinine. Plasma lipid profile, liver enzymes, urea and creatinine were determined using enzymatic end point method under standard operating procedures. Statistical analysis was done using Graph Pad Prism Version 5.03 and p values less than 0.05 were considered statistically significant.

Results: Results revealed that the STZ-induced diabetic group exhibited highly significant increase in activity of liver enzymes AST, ALT and ALP, increase in the levels of urea, creatinine, glucose and most lipid profile parameters as compared to the negative control group (p<0.001). Histopathological examination of liver and kidney tissues of diabetic rats indicated slight changes. However, their changes were overcame by clove treatment and the majority of the cells tend to be normal. Low dose clove group 5 (250 mg/kg) with metformin decreased the levels of the analytes most when compared to the levels of the positive control group. For glucose, group 5 gave a mean glucose level of 4.40 ± 1.08 mmo/l, significantly lower than the positive control group 39.67 ± 0.67, ALT group 5 gave a mean ALT level of 56.00 ± 7.11, which was significantly lower than the positive control group 205.70 ± 14.79, for urea group 5 gave a mean urea level of 4.25 ± 0.77 which was significantly lower the control group 23.80 ± 3.56  at  p < 0.001, thereby yielding a better treatment result.

Conclusion: In conclusion, low dose clove supplementation with metformin could be excellent adjuvant support in the therapy of diabetes mellitus and its complications.

Aim: Till now, no specific treatment is available for COVID-19. This work is carried out with the aim of verifying in the literature the antiviral properties of aromatic plants and essential oils that can justify their use against the causative agent of COVID-19, SARS-CoV-2.

Methodology: The literature review was based mainly on the usual databases such as PubMed, PubMed Central., Science Direct, SCIELO, DOAJ, Science alert, Semantic scholar and Google scholar.

Results: A survey of literature reveals that aromatic plants and their essential oils are active against a large number of viruses (Herpes virus-1, Herpes virus-2, HIV, Adeno virus, Hepatite B Virus, Enterovirus 71, JUNV, etc.) and even against SARS-CoV-1 which has 96% of the same genetic background with SARS-CoV-2.

Conclusion: Aromatic plants and their essential oils exhibit high antiviral activities against several types of viruses. This evidence stemming from several experimental studies means that some compounds derived from essential oils could act as inhibitors of COVID-19.

Molecular docking investigations and pharmacoinformatics of some compounds derived from essential oils with SARS-CoV-2 protease are in progress to identify the potential inhibitors of the virus.