Open Access Original Research Article

The Investigation of Antimutagenic Effects on Chromosomes and Cell Division Mechanisms against Mitomycine C in Human Lymphocyte Culture of Liquid Extracts Obtained from Blueberry (Vaccinium myrtillus L.) and Raspberry (Rubus idaeus L.)

Müge Mavioğlu Kaya, Süleyman Gül

Journal of Complementary and Alternative Medical Research, Page 1-14
DOI: 10.9734/jocamr/2020/v12i230201

Aims: In this study, we determined that whether the liquid extracts of the above-ground parts of Vaccinium myrtillus L. and Rubus idaeus L. have antimutagenic effects against mitomycin C in human peripheral lymphocytes, chromosome aberration (CA), micronucleus (MN) and mitotic index (MI) tests.

Methodology: Blood samples of the negative control group (Group I) were allowed to react in a cell culture medium without any treatment, while positive control group (Group II) was allowed to interact in a cell culture medium supplemented with mitomycin-C (MMC) at a dose of 0.3 μL /mL for each chromosome medium. Blood samples of the other groups were allowed to react for 24 hours with 0.3 μL /mL MMC and V. myrtillus L. (Group III, IV, V and VI), and R. idaeus L. (VII, VIII, IX and X) extracts in 0.2 μL /mL, 0.4 μL /mL, 0.8 μL /mL and 1.6 μL /mL doses for each of cell cultures. At the end of the incubation period, culture cells were evaluated by chromosomal aberrations, mitotic, micronucleus and nuclear division index tests.

Results: Compared with Group II, it was determined that mitotic, nuclear division and nuclear cytotoxic cleavage indices were increased when chromosome aberrations and micronucleus indexes were decreased in extract groups. When we compared to extract groups and group II, we observed that chromosome aberrations and micronucleus index decreased in extract groups, while mitotic, nuclear division and nuclear cytotoxic cleavage indices were increased.

Conclusion: It was concluded that aqueous extracts of V. myrtillus L. and Rubus idaeus L. had significantly antimutagenic effects on the human peripheral lymphocyte cells at the working doses.

Open Access Original Research Article

Conventional Ginger Candy on Premenstural Syndrome among Adolescent Girls

G. Bhuvaneswari, S. Gnanamalar, M. Jeevarathinam, S.Gayathri Devi

Journal of Complementary and Alternative Medical Research, Page 15-20
DOI: 10.9734/jocamr/2020/v12i230202

Background: Premenstrual condition is one of the most well-known issues in ladies at their regenerative age. Premenstrual disorder is a condition that influences the feelings, wellbeing, and conduct during specific days of the feminine cycle before her menses. Almost 80% of ladies report at least one manifestation that doesn't generously influence every day functioning, as per the American Family Doctor. In this aspect the current examination was led with the expection to decide the impact of regular ginger candy on premenstrual disorder among young ladies.

Methods: Quasi experimental research design was adopted with 60 samples who met the inclusion criteria. Samples were selected by e purposive sampling technique. Pre-test was conducted by using Numerical pain rating scale and HRQoL scale Questionnaire data was collected. Conventional ginger candy was administered to adolescent girls with routine daily diet. Posttest was conducted after 8 weeks with the same tool. Data were analyzed using SPSS. The Results: The study findings revealed that the severity of Premenstrual pain was reduced compared to before the intervention. HRQoL also improve routine Conventional ginger candy supplementation was effective in reducing the Premenstrual syndrome at the level of p>0.001 in the after the post test.

Conclusion: Conventional ginger candy supplementation is cost effective and easily available, thereby finding can relieve premenstrual pain and associated symptoms, and improve the quality of life among adolescent girls.

Open Access Original Research Article

Identification of People at Risk of Developing Chronic Kidney Disease among Rural Disadvantageous Population in Bangladesh

Tanjina Rahman, Akibul Islam Chowdhury, Mohammad Asadul Habib, Harun Ur- Rashid, Shakib Arefin, Zannatul Ferdous Shashi, Aidah Tasnim

Journal of Complementary and Alternative Medical Research, Page 21-29
DOI: 10.9734/jocamr/2020/v12i230204

Background: Chronic kidney disease (CKD) is a global disease and the prevalence of CKD is increasing in both developed and developing countries. The current study aimed to assess subjects in the rural areas of Sylhet district in Bangladesh to identify individuals who may be predisposed to at risk for developing CKD.

Methods: A cross-sectional study was carried out among 996 subjects from Sylhet district of Bangladesh. Data were collected by using a standard questionnaire from 82 villages. Data about socio-demographic, medical history and anthropometric and biochemical parameters were collected. Urine dipstick test was done for both albumin and glucose. Descriptive statistics and ANOVA-test were performed for statistical analysis.

Results: The study revealed that people living in rural areas of Sylhet in Bangladesh are at risk of developing CKD and the hidden cause behind it includes not only diabetes and hypertension, but also other lifestyle related factors. Younger participants were found to be at less risk compared to older participants for developing CKD. From urinary dipstick test, 2% and 3.3% subjects had severe traces of albumin and glucose in their urine. Approximately 16% of subjects had hypertension. From the data of 99 out of 996 subjects for urine albumin dipstick test, 98 respondents were identified as stage I CKD patients and only one was identified as stage II CKD patients.

Conclusion: As dialysis and transplants are unsustainable in the long term, it is important to seek preventive strategies when patients are in pre-dialysis state and identify and manage those at high risk. Nutrition and life-style choices can play key roles to achieve this. So, urgent low-cost programs are needed to identify people who are at risk of CKD as well as address their current medical condition to initiate early management of CKD patients.

Open Access Original Research Article

Public Perceptions of Cupping Therapy (Hijama) and Whether It Will Be Chosen Over Donating Blood

Abdulraheem Alshareef, Abdulrahman Amer Albeladi, Ahmad Khalaf Alsaedi, Ahmad Abdulaziz Alnakhli, Raed Saad AlHejili

Journal of Complementary and Alternative Medical Research, Page 30-35
DOI: 10.9734/jocamr/2020/v12i230205

Background: Modern medicine, despite its great advances, still not as effective as cupping therapy in treating many medical conditions.

Objective: To assess the level of awareness and the general perceptions about cupping therapy (Hijama) in the Saudi population.

Methods: We conducted a cross-sectional study among 1120 adult subjects (473 males and 647 females), during the period from January to March 2020. Participants responded to an anonymous self-administered questionnaire requesting information about their knowledge, attitude, and perceptions of cupping therapy. The data collected from 1120 questionnaires were analyzed using appropriate statistical methods in two stages (descriptive analysis - analysis of the study hypotheses). The frequencies and percentages of the descriptive analysis and the primary data of the study sample were calculated and the responses of its members to the questions included in the questionnaire were determined. The study hypotheses were analyzed using the chi-squared test to study the independence of the variables under study. The analysis was done using (IBM SPSS Statistics 25.0) software.

Results: About 32% of the participants underwent cupping before and the majority of them performed wet cupping (82.2%) and felt light pain (55.6%). Almost 60% of all participants were afraid of cupping and this fear was mainly from the injury (37%). The percentage of those who prefer to donate their blood was 72% while only 28% choose to perform cupping. There were statistically significant relationships between the gender of the participants and cupping procedure (p=0.003), fear of performing it (p<0.001) and preference for cupping over donating blood (p=0.002). Similarly, there were statistically significant relationships (p<0.001) between the age of the participants and cupping procedure, fear of performing it and preference for cupping over donating blood.

Conclusion: This study showed the high knowledge, attitudes and perception of the Saudi population towards wet cupping therapy. Importantly, most of the surveyed population, especially younger ones, choose to donate their blood rather than performing wet cupping. Further research is needed to establish a collaboration platform between wet cupping clinics and blood banks to fill the gap of frequent shortage of blood units.

Open Access Original Research Article

Nutritional Considerations, Lifestyle Changes and Smoking Habits in Geriatric Patients in COVID 19 ERA – Survey Report

Pragya Bali, Harvinder Singh, Yamini Ruthwal, Aditya Bali, Ashish Bali, Poonam Pathania, Farhat Jabeen

Journal of Complementary and Alternative Medical Research, Page 36-42
DOI: 10.9734/jocamr/2020/v12i230206

Aim: The main aim of this study is to investigate whether or not there has been a change in the daily dietary patterns of elderly people between the ages of  60 and 80 during the Coronavirus quarantine.

Materials and Methods: An online survey was conducted with questionnaires related to dietary habits and changes done in COVID 19 in elderly patients. An online form was sent with no physical contacts and questions were asked related to dietary habits in age group 60-80 years. Questions were related to Daily diet, Systematic diseases and mental health.

Results: With regards to lifestyle changes during the COVID-19 lockdown, most of the population declares not to have changed its habits. 48% of population has not changed their lifestyle where as 16% has improved their nutritional intake and 36% have witnessed decline their dietary habits during pandemic and lockdown.

Conclusion: The three pillars which should be controlled in COVID 19 times has been taken under and an insight is provided. COVID 19’s peculiarity is it enhances the chronic respiratory ailment in geriatric group hence diet plays an important role.